Anticorps Monoclonal anti-ING2

ING2 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C3A7

N° de cat : 66512-1-Ig

Synonymes

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

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Galerie de données de validation

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  WB analysis of HSC-T6 using 66512-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66512-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66512-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 66512-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human ING2 (66512-1-Ig, Clone:2C3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HSCT6, cellules NIH/3T3, cellules T-47D
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66512-1-Ig cible ING2 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ING2 Protéine recombinante Ag19291
• Nom complet: inhibitor of growth family, member 2
• Masse moléculaire calculée: 280 aa, 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC030128
• Symbole du gène: ING2
• Identification du gène (NCBI): 3622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.