Anticorps Monoclonal anti-IL-4

• Phare
• Validé par KD/KO

IL-4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc et plus (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

37

CloneNo.

1G2C5

N° de cat : 66142-1-Ig

Synonymes

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1

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Galerie de données de validation

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  WB analysis of Jurkat using 66142-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig (IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 66142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of human breast cancer using 66142-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66142-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Ramos using 66142-1-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human IL-4 (66142-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,
• Résultats positifs en cytométrie: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below

Informations sur le produit

66142-1-Ig cible IL-4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Ag9940
• Nom complet: interleukin 4
• Masse moléculaire calculée: 153 aa, 17 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC070123
• Symbole du gène: IL4
• Identification du gène (NCBI): 3565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.; Authors - Shen Guo
rat	WB	Aging (Albany NY); Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis.; Authors - Zhenglan Han
rat	WB	Transl Stroke Res; Beneficial Effects of Theta-Burst Transcranial Magnetic Stimulation on Stroke Injury via Improving Neuronal Microenvironment and Mitochondrial Integrity.; Authors - Xuemei Zong
pig	WB	Front Cell Dev Biol; Intravenous AAV9 administration results in safe and widespread distribution of transgene in the brain of mini-pig; Authors - Yingqi Lin
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
rat	WB	Front Cell Dev Biol; DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.; Authors - Tingting Wang