Anticorps Monoclonal anti-IL-33

IL-33 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

2A9C7

N° de cat : 66235-1-Ig

Synonymes

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Galerie de données de validation

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  WB analysis of human lung using 66235-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66235-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 66235-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66235-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66235-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules hTERT-RPE1, tissu pulmonaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

66235-1-Ig cible IL-33 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-33 Protéine recombinante Ag21430
• Nom complet: interleukin 33
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC047085
• Symbole du gène: IL33
• Identification du gène (NCBI): 90865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publications

Species	Application	Title
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	IHC	Cell Cycle; Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis.; Authors - Xiaobo Long
human	WB,IHC	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
human	WB,IF	Cells; Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation.; Authors - Chia-Jung Kuo
mouse	WB,IF	Int J Mol Sci; An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation; Authors - Jufang Jia
human	WB,IHC,IF	Cell Death Dis; Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis; Authors - Qiong Wu