Anticorps Monoclonal anti-IL-17A
IL-17A Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, souris
Applications
WB, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
CloneNo.
1B3D5
N° de cat : 66148-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A549, cellules Daudi, cellules HL-60, cellules Jurkat, cellules MOLT-4, cellules Raji, cellules Ramos, cellules RAW 264.7, cellules U-937 |
Résultats positifs en IHC | tissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu de côlon humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF | tissu d'amygdalite humain, |
Résultats positifs en cytométrie | cellules Jurkat |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 11 publications below |
IHC | See 3 publications below |
FC | See 1 publications below |
Informations sur le produit
66148-1-Ig cible IL-17A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | IL-17A Protéine recombinante Ag3733 |
Nom complet | interleukin 17A |
Masse moléculaire calculée | 155 aa, 18 kDa |
Poids moléculaire observé | 18 kDa |
Numéro d’acquisition GenBank | BC067505 |
Symbole du gène | IL17A |
Identification du gène (NCBI) | 3605 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
Protocole
Product Specific Protocols | |
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WB protocol for IL-17A antibody 66148-1-Ig | Download protocol |
IHC protocol for IL-17A antibody 66148-1-Ig | Download protocol |
IF protocol for IL-17A antibody 66148-1-Ig | Download protocol |
FC protocol for IL-17A antibody 66148-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
J Integr Med Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9 | ||
Int Immunopharmacol (RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis. | ||
Int Immunopharmacol IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus. | ||
Biomed Pharmacother P-STAT3 and IL-17 in tumor tissues enhances the prognostic value of CEA and CA125 in patients with lung adenocarcinoma. | ||
J Ethnopharmacol Khasianine ameliorates psoriasis-like skin inflammation and represses TNF-α/NF-κB axis mediated transactivation of IL-17A and IL-33 in keratinocytes. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Rosa (Verified Customer) (07-08-2022) |
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