Anticorps Monoclonal anti-IL-17A

IL-17A Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3D5

N° de cat : 66148-1-Ig

Synonymes

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, interleukin 17A



Applications testées

Résultats positifs en WBcellules A549, cellules Daudi, cellules HL-60, cellules Jurkat, cellules MOLT-4, cellules Raji, cellules Ramos, cellules RAW 264.7, cellules U-937
Résultats positifs en IHCtissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu de côlon humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFtissu d'amygdalite humain,
Résultats positifs en cytométriecellules Jurkat

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66148-1-Ig cible IL-17A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène IL-17A Protéine recombinante Ag3733
Nom complet interleukin 17A
Masse moléculaire calculée 155 aa, 18 kDa
Poids moléculaire observé 18 kDa
Numéro d’acquisition GenBankBC067505
Symbole du gène IL17A
Identification du gène (NCBI) 3605
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

Protocole

Product Specific Protocols
WB protocol for IL-17A antibody 66148-1-IgDownload protocol
IHC protocol for IL-17A antibody 66148-1-IgDownload protocol
IF protocol for IL-17A antibody 66148-1-IgDownload protocol
FC protocol for IL-17A antibody 66148-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Nutr Food Res

A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways

Authors - Qianye Zhang
mouseIHC

J Integr Med

Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9

Authors - Ya Zhong
mouseWB

Int Immunopharmacol

(RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis.

Authors - Liangjun Deng
humanWB

Int Immunopharmacol

IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.

Authors - Yachun Li
humanIHC

Biomed Pharmacother

P-STAT3 and IL-17 in tumor tissues enhances the prognostic value of CEA and CA125 in patients with lung adenocarcinoma.

Authors - Xiao-Ke Chen
human,mouseWB,IHC

J Ethnopharmacol

Khasianine ameliorates psoriasis-like skin inflammation and represses TNF-α/NF-κB axis mediated transactivation of IL-17A and IL-33 in keratinocytes.

Authors - Yixi Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rosa (Verified Customer) (07-08-2022)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: adipose tissue
IL-17A Antibody Immunofluorescence validation (1:150 dilution) in adipose tissue (Cat no:66148-1-Ig)