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Anticorps Monoclonal anti-IL-6

IL-6 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

APC Fluorescent Dye

CloneNo.

4G3E6

N° de cat : APC-69001

Synonymes

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, Interferon beta 2, Interleukin 6

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HUVEC cells using APC-conjugated IL-6 Monoclonal antibody (APC-69001)

    FC experiment of HUVEC using APC-69001

    1X10^6 HUVEC cells untreated (dashed lines) or treated with LPS and Brefeldin A (red) were intracellularly stained with 0.2 ug APC Anti-Human NeutraKine® IL-6 (APC-69001, Clone:4G3E6) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • Flow cytometry (FC) experiment of HUVEC cells using APC-conjugated IL-6 Monoclonal antibody (APC-69001)

    FC experiment of HUVEC using APC-69001

    1X10^6 untrated or LPS and Brefeldin A treated HUVEC cells were intracellularly stained with 0.2 ug APC Anti-Human NeutraKine® IL-6 (APC-69001, Clone:4G3E6), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Applications testées

Résultats positifs en cytométriecellules HUVEC traitées au LPS et à la bréfeldine A

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-69001 cible IL-6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène IL-6 Protéine recombinante HZ-1019
Nom complet interleukin 6 (interferon, beta 2)
Symbole du gène IL6
Identification du gène (NCBI) 3569
Conjugaison APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.1% sodium azide and 0.5% BSA.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.