Anticorps Monoclonal anti-IL-4

IL-4 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

1

CloneNo.

11B11

N° de cat : PE-65074

Synonymes

Il 4, Il4, IL-4, interleukin 4

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Galerie de données de validation

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  FC experiment of C57BL/6 Th2-polarized splenocytes using PE-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th2-polarized splenocytes were surface stained 0.2 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.125 ug PE Anti-Mouse IL-4 (PE-65074, Clone: 11B11).

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin stimulated Th2-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

PE-65074 cible IL-4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Partially purified native mouse IL-4
• Nom complet: interleukin 4
• Numéro d’acquisition GenBank: BC027514
• Symbole du gène: Il4
• Identification du gène (NCBI): 16189
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Publications

Species	Application	Title
mouse	FC	J Ethnopharmacol; Daqing formula ameliorated allergic asthma and airway dysbacteriosis in mice challenged with ovalbumin and ampicillin; Authors - Li-Ren Wu