Anticorps Monoclonal anti-IL-4

IL-4 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

APC Fluorescent Dye

CloneNo.

11B11

N° de cat : APC-65074

Synonymes

Il 4, Il4, IL-4, interleukin 4

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Galerie de données de validation

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  FC experiment of C57BL/6 Th2-polarized splenocytes using APC-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) mouse Th2-polarized splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.5 ug APC Anti-Mouse IL-4 (APC-65074, Clone:11B11).

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin stimulated mouse Th2-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65074 cible IL-4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Partially purified native mouse IL-4
• Nom complet: interleukin 4
• Numéro d’acquisition GenBank: BC027514
• Symbole du gène: Il4
• Identification du gène (NCBI): 16189
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C