Anticorps Recombinant de lapin anti-IL-13

IL-13 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

2C14

N° de cat : 98003-1-RR

Synonymes

Il 13, Il13, interleukin 13

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Galerie de données de validation

• 

  FC experiment of C57BL/6 Th2-polarized splenocytes using 98003-1-RR

  1X10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.5 ug Anti-Mouse IL-13 (98003-1-RR, Clone:2C14) and Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, PE at dilution 1:666, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98003-1-RR cible IL-13 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 13
• Masse moléculaire calculée: 14 kDa
• Numéro d’acquisition GenBank: NM_008355
• Symbole du gène: Il13
• Identification du gène (NCBI): 16163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.