Anticorps Monoclonal anti-IL-12/IL-23 p40

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

PE Fluorescent Dye

CloneNo.

C17.8

N° de cat : PE-65097

Synonymes

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL-12/IL-23 p40, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

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Galerie de données de validation

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  FC experiment of Balb/c mouse peritoneal macrophages using PE-65097

  1X10^6 IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor or unstimulated were intracellularly stained with 0.5 ug PE Anti-Human IL-12/IL-23 p40 (PE-65097, Clone: C17.8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65097 cible IL-12/IL-23 p40 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-12 p70 recombinante de souris exprimée par CHO
• Nom complet: interleukin 12b
• Numéro d’acquisition GenBank: BC103608
• Symbole du gène: Il12b
• Identification du gène (NCBI): 16160
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C