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Anticorps Monoclonal anti-IL-12/IL-23 p40

IL-12/IL-23 p40 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

C17.8

N° de cat : CL647-65097

Synonymes

Il 12b, Il 12p40, IL-12/IL-23 p40, Il12b, Il-12b, Il12p40, interleukin 12b, p40

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (C17 (CL647-65097)

    FC experiment of mouse peritoneal macrophages using CL647-65097

    1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).

Applications testées

Résultats positifs en cytométrieIFN-γ primed murine peritoneal macrophages stimulated with LPS

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65097 cible IL-12/IL-23 p40 dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène IL-12 p70 recombinante de souris exprimée par CHO
Nom complet interleukin 12b
Numéro d’acquisition GenBankBC103608
Symbole du gène Il12b
Identification du gène (NCBI) 16160
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 IL-12/IL-23 p40 antibody CL647-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols