Anticorps Polyclonal de lapin anti-IL-1 Alpha

IL-1 Alpha Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB

Conjugaison

Non conjugué

N° de cat : 28475-1-AP

Synonymes

Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL1 ALPHA, IL-1 alpha, IL1A, IL-1A, IL1F1, Interleukin 1 alpha, interleukin 1, alpha

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Galerie de données de validation

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  WB analysis using 28475-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 28475-1-AP (IL-1 Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28475-1-AP cible IL-1 Alpha dans les applications de WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1 Alpha Protéine recombinante Ag29587
• Nom complet: interleukin 1, alpha
• Masse moléculaire calculée: 271 aa, 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013142
• Symbole du gène: IL1A
• Identification du gène (NCBI): 3552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.