Anticorps Monoclonal anti-IFT81

IFT81 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, Lapin, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1D5A3

N° de cat : 67987-1-Ig

Synonymes

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Galerie de données de validation

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  WB analysis using 67987-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 67987-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 67987-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (67987-1-Ig, Clone: 1D5A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules hTERT-RPE1, cellules MDCK, cellules NCCIT, tissu cérébral de lapin, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IF: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67987-1-Ig cible IFT81 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, Lapin, rat, souris

• Réactivité: canin, Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFT81 Protéine recombinante Ag31629
• Nom complet: intraflagellar transport 81 homolog (Chlamydomonas)
• Masse moléculaire calculée: 676 aa, 80 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC029349
• Symbole du gène: IFT81
• Identification du gène (NCBI): 28981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.