Anticorps Polyclonal de lapin anti-IFT74

• Phare
• Validé par KD/KO

IFT74 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 27334-1-AP

Synonymes

CCDC2, CMG 1, CMG1, IFT74

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 27334-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27334-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 27334-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules Jurkat, cellules Raji, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

27334-1-AP cible IFT74 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT74 Protéine recombinante Ag26385
• Nom complet: intraflagellar transport 74 homolog (Chlamydomonas)
• Masse moléculaire calculée: 600 aa, 69 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC107742
• Symbole du gène: IFT74
• Identification du gène (NCBI): 80173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Genet Med; Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.; Authors - Minna Luo ; KD Validated
human	WB	Pulm Circ; Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia; Authors - Saejeong Park
human,mouse	IF	Hum Reprod; Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility; Authors - Qi Li
human	WB,IP	Hum Mol Genet; Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations; Authors - Mahmoud R Fassad
human	WB	EMBO J; The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport; Authors - Niels Boegholm ; KO Validated
mouse	WB,CoIP	EMBO Mol Med; IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect; Authors - Jing Dai