Anticorps Monoclonal anti-IFN gamma

IFN gamma Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

PE Fluorescent Dye

Publications

5

CloneNo.

XMG1.2

N° de cat : PE-65153

Synonymes

Ifg, IFN g, IFN gamma, Ifng, IFN-gamma, IFNγ, IFN-γ, interferon gamma

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65153

  1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65153 cible IFN gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFN-γ recombinant de souris exprimé par E. coli
• Nom complet: interferon gamma
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC119063
• Symbole du gène: Ifng
• Identification du gène (NCBI): 15978
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Publications

Species	Application	Title
mouse	FC (Intra)	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
	FC (Intra)	Nat Commun; CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis; Authors - Ping Yang
mouse	FC (Intra)	ACS Nano; Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity; Authors - Yunkai Shang
mouse	FC (Intra)	Cell Mol Gastroenterol Hepatol; Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation; Authors - Shan Fu
mouse	FC (Intra)	J Med Chem; Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer; Authors - Linxiang Cai

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C