Anticorps Monoclonal anti-IFN gamma

IFN gamma Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

XMG1.2

N° de cat : FITC-65153

Synonymes

Ifg, IFN g, IFN gamma, IFN γ, Ifng, IFN-gamma, IFN-γ, interferon gamma

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Galerie de données de validation

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  FC experiment of C57BL/6 Th1-polarized splenocytes using FITC-65153

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of Brefeldin A and Monensin) Th1-polarized splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug FITC Plus Anti-Mouse IFN gamma (FITC-65153, Clone: XMG1.2).

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin stimulated Th1-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65153 cible IFN gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFN-γ recombinant de souris exprimé par E. coli
• Nom complet: interferon gamma
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC119063
• Symbole du gène: Ifng
• Identification du gène (NCBI): 15978
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Publications

Species	Application	Title
mouse	FC (Intra)	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng