Anticorps Monoclonal anti-IFITM2/3

• Phare
• Validé par KD/KO

IFITM2/3 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

2E8D12

N° de cat : CL555-66081

Synonymes

1 8U, IFITM3, IP15

---

Galerie de données de validation

• 

  IF Staining of U2OS using CL555-66081

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®555 IFITM2/3 antibody (CL555-66081, Clone: 2E8D12 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL555-66081

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IFITM2/3 (CL555-66081, Clone:2E8D12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: cellules U2OS,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-66081 cible IFITM2/3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFITM2/3 Protéine recombinante Ag17863
• Nom complet: interferon induced transmembrane protein 3 (1-8U)
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC070243
• Symbole du gène: IFITM3
• Identification du gène (NCBI): 10410
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C