Anticorps Monoclonal anti-ICAM2

ICAM2 Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, FC,ELISA

Conjugaison

Non conjugué

CloneNo.

1H3C6

N° de cat : 67628-1-Ig

Synonymes

CD102, ICAM 2, ICAM2

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 67628-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67628-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 67628-1-Ig

  1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (67628-1-Ig, Clone:1H3C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mouse IgG2a isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules Jurkat
• Résultats positifs en cytométrie: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67628-1-Ig cible ICAM2 dans les applications de WB, FC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICAM2 Protéine recombinante Ag29441
• Nom complet: intercellular adhesion molecule 2
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC003097
• Symbole du gène: ICAM2
• Identification du gène (NCBI): 3384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.