Anticorps Polyclonal de lapin anti-ICAM2

ICAM2 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 27322-1-AP

Synonymes

CD102, ICAM 2, ICAM2

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Galerie de données de validation

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  WB analysis using 27322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 27322-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27322-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 27322-1-AP

  1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (27322-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HUVEC, cellules Jurkat, tissu pulmonaire de souris
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27322-1-AP cible ICAM2 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ICAM2 Protéine recombinante Ag25952
• Nom complet: intercellular adhesion molecule 2
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 55-80 kDa
• Numéro d’acquisition GenBank: BC003097
• Symbole du gène: ICAM2
• Identification du gène (NCBI): 3384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.