Anticorps Monoclonal anti-ICAM-1

• Phare
• Validé par KD/KO

ICAM-1 Monoclonal Antibody for FC, IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F9A8

N° de cat : CL488-60299

Synonymes

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Galerie de données de validation

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  IF Staining of human lung cancer using CL488-60299

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL488-60299 (ICAM-1 antibody) at dilution of 1:100.

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  IF Staining of human lung cancer using CL488-60299

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL488-60299 (ICAM-1 antibody) at dilution of 1:100.

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  FC experiment of Raji using CL488-60299

  1X10^6 Raji cells were surface stained with 0.8 ug CoraLite® Plus 488 Anti-Human ICAM-1 (CL488-60299, Clone:2F9A8) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF: tissu de cancer du poumon humain,
• Résultats positifs en cytométrie: cellules Raji,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60299 cible ICAM-1 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICAM-1 Protéine recombinante Ag8309
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC015969
• Symbole du gène: ICAM1
• Identification du gène (NCBI): 3383
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).