• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Monoclonal anti-IBA1

IBA1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

IF

Conjugaison

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1C6A10

N° de cat : CL594-66827

Synonymes

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 594-conjugated IBA1 Monoclonal anti (CL594-66827)

    IF Staining of mouse brain using CL594-66827

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66827 (IBA1 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 594-conjugated IBA1 Monoclonal anti (CL594-66827)

    IF Staining of mouse brain using CL594-66827

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66827 (IBA1 antibody) at dilution of 1:50.

Applications testées

Résultats positifs en IFtissu cérébral de souris,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

IFSee 1 publications below

Informations sur le produit

CL594-66827 cible IBA1 dans les applications de IF et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéesouris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène IBA1 Protéine recombinante Ag28236
Nom complet allograft inflammatory factor 1
Masse moléculaire calculée 17 kDa
Numéro d’acquisition GenBankBC009474
Symbole du gène AIF1
Identification du gène (NCBI) 199
Conjugaison CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.

Publications

SpeciesApplicationTitle
mouseIF

Brain Behav Immun

Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction.

Authors - Chan Chen
View Article