- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-IBA1
IBA1 Polyclonal Antibody for IF
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
IF
Conjugaison
CoraLite®594 Fluorescent Dye
N° de cat : CL594-10904
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF | tissu cérébral de rat, BV-2 cells |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| IF | See 1 publications below |
Informations sur le produit
CL594-10904 cible IBA1 dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | IBA1 Protéine recombinante Ag1363 |
| Nom complet | allograft inflammatory factor 1 |
| Masse moléculaire calculée | 17 kDa |
| Numéro d’acquisition GenBank | BC009474 |
| Symbole du gène | AIF1 |
| Identification du gène (NCBI) | 199 |
| Conjugaison | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
What is the molecular weight of IBA1?
The molecular weight of IBA1 is 16.7 kD.
What is IBA1?
Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).
What is the function of IBA1?
IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).
Where is IBA1 localized?
IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).
Is IBA1 upregulated in active immunophages?
Yes; its expression is associated with inflammatory activity (PMID: 29749461).
How do IBA1-positive microglia differ from microglia that express less or no IBA1?
'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).
How is IBA1 related to Alzheimer's disease and pain?
Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 IBA1 antibody CL594-10904 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Immunol QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH rajkumar (Verified Customer) (02-27-2023) | The Coralite 594 iba-1 antibody worked perfectly in the primary isolated microglial cells and also in the brain tissue.
|
