Anticorps Monoclonal anti-HuR/ELAVL1

• Phare
• Validé par KD/KO

HuR/ELAVL1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

1A11H2

N° de cat : 66549-1-Ig

Synonymes

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

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Galerie de données de validation

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  WB analysis using 66549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 66549-1-Ig

  WB result of HuR/ELAVL1 antibody (66549-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells.

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  WB analysis using 66549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66549-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HuR/ELAVL1 antibody (66549-1-Ig, Clone: 1A11H2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NCI-H1299, cellules Neuro-2a, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

66549-1-Ig cible HuR/ELAVL1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HuR/ELAVL1 Protéine recombinante Ag2249
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 34-37 kDa
• Numéro d’acquisition GenBank: BC003376
• Symbole du gène: ELAVL1
• Identification du gène (NCBI): 1994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
mouse	WB,IF	Mol Cell Endocrinol; ELAV-like RNA binding protein 1 regulates osteogenesis in diabetic osteoporosis: Involvement of divalent metal transporter 1.; Authors - Yuanfei Ren
human	WB	Cell Signal; Butyrate mediated regulation of RNA binding proteins in the post-transcriptional regulation of inflammatory gene expression.; Authors - Aydan Torun
human	WB	J Int Med Res; Long non-coding RNA DEPDC1-AS1 promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R-F11R pathway.; Authors - Wei Xu
human	WB	Cell Death Dis; LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis; Authors - Deji Ye ; KD Validated
human	IF	Oncol Lett; Stress granules affect the sensitivity of renal cancer cells to sorafenib by sequestering and stabilizing COX‑2 mRNA; Authors - Huiqi Dai