Anticorps Monoclonal anti-HSPB3

HSPB3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A10B5

N° de cat : 67890-1-Ig

Synonymes

Heat shock 17 kDa protein, heat shock 27kDa protein 3, Heat shock protein beta 3, HSP 17, HSPB3, HSPL27

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Galerie de données de validation

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  WB analysis using 67890-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human heart using 67890-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 67890-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 67890-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67890-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67890-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67890-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human HSPB3 (67890-1-Ig, Clone:2A10B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu cardiaque de lapin, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67890-1-Ig cible HSPB3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPB3 Protéine recombinante Ag26940
• Nom complet: heat shock 27kDa protein 3
• Masse moléculaire calculée: 150 aa, 17 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC126272
• Symbole du gène: HSPB3
• Identification du gène (NCBI): 8988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.