Anticorps Monoclonal anti-HSPA2

HSPA2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1A4A9

N° de cat : 66291-1-Ig

Synonymes

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Galerie de données de validation

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  WB analysis using 66291-1-Ig

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66291-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66291-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

66291-1-Ig cible HSPA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPA2 Protéine recombinante Ag24228
• Nom complet: heat shock 70kDa protein 2
• Masse moléculaire calculée: 639 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC036107
• Symbole du gène: HSPA2
• Identification du gène (NCBI): 3306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Publications

Species	Application	Title
human	WB	Clin Epigenetics; Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations.; Authors - J Pei
human	WB	Front Cardiovasc Med; Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy.; Authors - Maike Schuldt
human	WB	Cells; Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy.; Authors - Larissa M Dorsch