Anticorps Monoclonal anti-HSP90

HSP90 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

64

CloneNo.

3F11C1

Avis (2)

N° de cat : 60318-1-Ig

Synonymes

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

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Galerie de données de validation

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  WB analysis using 60318-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  IP experiment of HepG2 using 60318-1-Ig

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug.

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  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60318-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of K-562 using 60318-1-Ig

  1X10^6 K-562 cells were stained with 0.2ug HSP90 antibody (60318-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules ROS1728
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 61 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

60318-1-Ig cible HSP90 dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag3826
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Masse moléculaire calculée: 853 aa, 90 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC023006
• Symbole du gène: HSP90AA1
• Identification du gène (NCBI): 3320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse,human	WB	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu
human	WB	Aging Cell; Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis.; Authors - Davide D'Amico
human	WB,IP,RIP	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
	WB	Cell Death Dis; HOXC11 drives lung adenocarcinoma progression through transcriptional regulation of SPHK1; Authors - Xin Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 90 kd, as shown in the website.

• Applications: Western Blot

FH

Ayyappan (Verified Customer) (01-16-2019)

This antibody works well for IP in human cancer cell lines

• Applications: Immunoprecipitation,