Anticorps Polyclonal de lapin anti-HO-1/HMOX1

HO-1/HMOX1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

123

N° de cat : 27282-1-AP

Synonymes

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Galerie de données de validation

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  WB analysis of HeLa using 27282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27282-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27282-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 27282-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, tissu splénique de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 122 publications below
• IHC: See 7 publications below
• IF: See 5 publications below

Informations sur le produit

27282-1-AP cible HO-1/HMOX1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HO-1/HMOX1 Protéine recombinante Ag26172
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001491
• Symbole du gène: HMOX1
• Identification du gène (NCBI): 3162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang
human	WB	ACS Appl Mater Interfaces; Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration.; Authors - Zhao Xu
human,rat	WB	Free Radic Biol Med; Neuroprotective effect of phosphocreatine on oxidative stress and mitochondrial dysfunction induced apoptosis in vitro and in vivo: Involvement of dual PI3K/Akt and Nrf2/HO-1 pathways.; Authors - Hailong Li
mouse	WB	Biomed Pharmacother; Qing brick tea (QBT) aqueous extract protects monosodium glutamate-induced obese mice against metabolic syndrome and involves up-regulation Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) antioxidant pathway.; Authors - Wenqi Gao
rat	WB,IF	Oxid Med Cell Longev; Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia.; Authors - Liren Zhong