Anticorps Monoclonal anti-HNRNPF

• Phare
• Validé par KD/KO

HNRNPF Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2E7A4

N° de cat : 67701-1-Ig

Synonymes

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

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Galerie de données de validation

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  WB analysis using 67701-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67701-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67701-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

67701-1-Ig cible HNRNPF dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPF Protéine recombinante Ag7128
• Nom complet: heterogeneous nuclear ribonucleoprotein F
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC001432
• Symbole du gène: HNRNPF
• Identification du gène (NCBI): 3185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Publications

Species	Application	Title
mouse	WB	Cell Rep; mTOR inhibition reprograms cellular proteostasis by regulating eIF3D-mediated selective mRNA translation and promotes cell phenotype switching; Authors - Sejeong Shin ; KD Validated
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	RNA Biol; hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.; Authors - Tanzina Tanu ; KD Validated
mouse	WB	Asian J Androl; Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis; Authors - Shui-Qiao Q Yuan