Anticorps Polyclonal de lapin anti-HNF4G

• Phare
• Validé par KD/KO

HNF4G Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, chIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25801-1-AP

Synonymes

HNF 4 gamma, HNF4G, NR2A2, NR2A3

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Galerie de données de validation

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  WB analysis of mouse pancreas using 25801-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25801-1-AP (HNF4G Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25801-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25801-1-AP (HNF4G Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 25801-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug HNF4G antibody (25801-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu rénal de souris
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

25801-1-AP cible HNF4G dans les applications de WB, IHC, FC, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNF4G Protéine recombinante Ag22848
• Nom complet: hepatocyte nuclear factor 4, gamma
• Masse moléculaire calculée: 445 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC105009
• Symbole du gène: HNF4G
• Identification du gène (NCBI): 3174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNF4 (hepatocyte nuclear factor 4) is a nuclear receptor protein that has a critical role in hepatocyte differentiation and function (PMID: 12774000). Two isoforms of human HNF4 exist, HNF4-alpha and HNF4-gamma, which are encoded by two separate genes HNF4A and HNF4G respectively (PMID: 7926813). HNF4-gamma has a lower transcription activation potential than HNF4-alpha. HNF4-gamma is expressed in kidney, gut, pancreas, and testis (PMID: 16945327). It has been reported to be involved in cancer progression (PMID: 23896584). This polyclonal antibody is raised against C-terminal 82 amino acids of human HNF4-gamma.

Publications

Species	Application	Title
mouse	chIP	Cancer Cell; Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance.; Authors - Shipra Shukla
human	WB,IHC	Protein Cell; Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation.; Authors - Chengcheng Wang
human	WB,IHC	Eur J Pharmacol; Hepatocyte nuclear factor 4 gamma (HNF4G) is correlated with poor prognosis and promotes tumor cell growth by inhibiting caspase-dependent intrinsic apoptosis in colorectal cancer.; Authors - Lu Chen ; KD Validated
human	chIP	Clin Exp Metastasis; Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.; Authors - Yahui Huang
human	WB,ChIP	Clin Transl Oncol; HNF4G stimulates the development of pancreatic cancer by promoting IGF2BP2 transcription; Authors - Jian Zhan ; KD Validated
human,mouse	WB	Cell Rep; MFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4γ-dependent transcription of CES enzymes; Authors - Ritwik Datta ; KO Validated