- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-HMGB3
HMGB3 Polyclonal Antibody for IF, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 27465-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules BGC-823, cellules MCF-7, cellules SGC-7901, tissu placentaire humain |
| Résultats positifs en IF | cellules MCF-7, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
Informations sur le produit
27465-1-AP cible HMGB3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | HMGB3 Protéine recombinante Ag26361 |
| Nom complet | high-mobility group box 3 |
| Masse moléculaire calculée | 200 aa, 23 kDa |
| Poids moléculaire observé | 25 kDa |
| Numéro d’acquisition GenBank | BC070482 |
| Symbole du gène | HMGB3 |
| Identification du gène (NCBI) | 3149 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
HMGB3, also named HMG2A and HMG4, belongs to the HMGB family. HMGB3 binds to nucleosomes in a sequence-independent manner and participates in DNA repair, replication, transcription, and recombination. HMGB3 is highly expressed in stem cells and cancer cells and is rarely transactivated in normal adult tissues, making it a promising therapeutic target. Overexpressed HMGB3 is closely related to tumor occurrence and reduced survival in cervical cancer, non-small-cell lung cancer, breast cancer, bladder cancer, hepatocellular carcnoma, colorectal cancer, gastric cancer, and leukemia (PMID: 35332131).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGB3 antibody 27465-1-AP | Download protocol |
| IF protocol for HMGB3 antibody 27465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol HMGB3 is a Potential Therapeutic Target by Affecting the Migration and Proliferation of Colorectal Cancer.
| ||
Genes (Basel) Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.
| ||
Int J Gen Med Circular RNA 0001313 Knockdown Suppresses Non-Small Cell Lung Cancer Cell Proliferation and Invasion via the microRNA-452/HMGB3/ERK/MAPK Axis. | ||
Front Oncol Loss of exosomal micro-RNA-200b-3p from hypoxia cancer-associated fibroblasts reduces sensitivity to 5-flourouracil in colorectal cancer through targeting high-mobility group box 3 | ||
Curr Med Sci Overexpression of MiR-633 Suppresses the Tumorigenicity of Gastric Cancer Cells and Induces Apoptosis by Targeting MAPK1 |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ting (Verified Customer) (10-18-2023) | The Antibody looked bad. I saw many non-specific bands and two bands near the HMGB3 molecular weight when I use this antibody. It is really hard to distinguish the bands, especially for Wild-type group.
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