Anticorps Monoclonal anti-HMGB1

HMGB1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

CloneNo.

1D12A6

N° de cat : 66525-1-Ig

Synonymes

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

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Galerie de données de validation

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  WB analysis using 66525-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66525-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66525-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66525-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, cellules K-562, cellules L02, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

66525-1-Ig cible HMGB1 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HMGB1 Protéine recombinante Ag1264
• Nom complet: high-mobility group box 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003378
• Symbole du gène: HMGB1
• Identification du gène (NCBI): 3146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Nat Microbiol; Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis; Authors - Tomas Doyle
mouse	WB	Adv Healthc Mater; Anti-PD-L1 DNAzyme Loaded Photothermal Mn 2+ /Fe 3+ Hybrid Metal-Phenolic Networks for Cyclically Amplified Tumor Ferroptosis-Immunotherapy; Authors - Peng Liu
mouse		ACS Appl Mater Interfaces; Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy; Authors - Junmei Zhang
human,mouse	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
mouse	WB	Oxid Med Cell Longev; The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.; Authors - Yichao Du