Anticorps Monoclonal anti-HMG20A

HMG20A Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

1B10F12

N° de cat : 66676-1-Ig

Synonymes

FLJ10739, high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

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Galerie de données de validation

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  WB analysis of HeLa using 66676-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66676-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66676-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66676-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66676-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellule NCCIT, cellules HEK-293, cellules Jurkat
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66676-1-Ig cible HMG20A dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HMG20A Protéine recombinante Ag2718
• Nom complet: high-mobility group 20A
• Masse moléculaire calculée: 347 aa, 40 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC021959
• Symbole du gène: HMG20A
• Identification du gène (NCBI): 10363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation