Anticorps Polyclonal de lapin anti-HLA-E

• Phare
• Validé par KD/KO

HLA-E Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 27411-1-AP

Synonymes

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

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Galerie de données de validation

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  WB analysis of THP-1 using 27411-1-AP

  WB result of HLA-E antibody (27411-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

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  WB analysis of human placenta using 27411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27411-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 27411-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 27411-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HL-60, cellules MDA-MB-231, cellules THP-1, cellules U-937
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27411-1-AP cible HLA-E dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-E Protéine recombinante Ag26581
• Nom complet: major histocompatibility complex, class I, E
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC002578
• Symbole du gène: HLA-E
• Identification du gène (NCBI): 3133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)