Anticorps Polyclonal de lapin anti-Di-Methyl-Histone H3 (Lys36)

Di-Methyl-Histone H3 (Lys36) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 29202-1-AP

Synonymes

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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Galerie de données de validation

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  WB analysis using 29202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (HIST1H3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (Di-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of peptide using 29202-1-AP

  Di-Methyl-Histone H3(Lys36) antibody (29202-1-AP) tested by ELISA. HIST1H3A(31-43aa), HIST1H3AMe1(31-43aa), HIST1H3AMe2(31-43aa), HIST1H3AMe3(31-43aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of HIST1H3AMe2 antibody (29202-1-AP). Bound antibodies were detected with HRP conjugated anti-rabbit IgG followed by incubation with HRP substrate and then measuring the resulting absorbance at 450 nm.

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules HEK-293T, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules THP-1
• Résultats positifs en ELISA: Peptide,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Test immuno-enzymatique (ELISA): ELISA : 1:32000-1:128000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29202-1-AP cible Di-Methyl-Histone H3 (Lys36) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).