Anticorps Monoclonal anti-HIF-1 alpha

HIF-1 alpha Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1H3C12

N° de cat : 66730-1-Ig

Synonymes

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8



Applications testées

Résultats positifs en WBcellules HeLa, cellules HeLa traitées au chlorure de cobalt, cellules HepG2 traitées au chlorure de cobalt

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66730-1-Ig cible HIF-1 alpha dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène HIF-1 alpha Protéine recombinante Ag15198
Nom complet hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Masse moléculaire calculée 826 aa, 93 kDa
Poids moléculaire observé 120 kDa
Numéro d’acquisition GenBankBC012527
Symbole du gène HIF1A
Identification du gène (NCBI) 3091
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protocole

Product Specific Protocols
WB protocol for HIF-1 alpha antibody 66730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Adv Sci (Weinh)

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Authors - Huan Li
humanWB

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu
humanWB

Theranostics

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Authors - Xiaoxin Zhang
humanWB

Oxid Med Cell Longev

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.

Authors - Ziwei Yang
humanWB

J Med Chem

Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2.

Authors - Xuan Qin
humanWB,IHC

J Clin Endocrinol Metab

Targeting tumor hypoxia inhibits aggressive phenotype of dedifferentiated thyroid cancer

Authors - Ben Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: renal Cancer cell line
FH

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1250
  • Cell Tissue Type: Cell lysates glioblastoma primary cells
HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)