Anticorps Monoclonal anti-HDAC4

HDAC4 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

CloneNo.

1D2G12

N° de cat : 66838-1-Ig

Synonymes

HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288



Applications testées

Résultats positifs en WBcellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7
Résultats positifs en IHCtissu de cancer du sein humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochimie (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66838-1-Ig cible HDAC4 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène HDAC4 Protéine recombinante Ag11345
Nom complet histone deacetylase 4
Masse moléculaire calculée 972 aa, 106 kDa
Poids moléculaire observé 140 kDa
Numéro d’acquisition GenBankBC039904
Symbole du gène HDAC4
Identification du gène (NCBI) 9759
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Protocole

Product Specific Protocols
WB protocol for HDAC4 antibody 66838-1-IgDownload protocol
IHC protocol for HDAC4 antibody 66838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan
humanIF

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanIF

J Mol Cell Cardiol

Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.

Authors - Yongyun Li
mouseWB,IP,ChIP

Appl Physiol Nutr Metab

Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle

Authors - Song Huang
  • KD Validated
mouseWB

J Toxicol Sci

Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS

Authors - Jia Luo
  • KD Validated
human,mouseWB

Adv Clin Exp Med

Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway

Authors - Ying Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

In our screening of basic expression in lung, breast, and gastric cancer cell lines, antibody concentrations ranging from 1:1000 to 2000 all displayed clear bands, indicating stable working conditions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Multiple cell lines of lung cancer, breast cancer, gastric cancer.
HDAC4 Antibody Western Blot validation (1:1000 dilution) in Multiple cell lines of lung cancer, breast cancer, gastric cancer. (Cat no:66838-1-Ig)