Anticorps Monoclonal anti-HDAC2

• Phare
• Validé par KD/KO

HDAC2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1A3E4

Avis (1)

N° de cat : 67165-1-Ig

Synonymes

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1

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Galerie de données de validation

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  WB analysis of MCF-7 using 67165-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67165-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67165-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67165-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67165-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (67165-1-Ig, Clone: 1A3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 67165-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HDAC2 (67165-1-Ig, Clone:1A3E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2, cellules Jurkat, cellules NCCIT
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67165-1-Ig cible HDAC2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC2 Protéine recombinante Ag21288
• Nom complet: histone deacetylase 2
• Masse moléculaire calculée: 458 aa, 52 kDa; 488 aa,55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC031055
• Symbole du gène: HDAC2
• Identification du gène (NCBI): 3066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.

Publications

Species	Application	Title
human	WB,CoIP	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang ; KD Validated
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan
human,rat	WB,IF,IP	Toxicol Appl Pharmacol; Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.; Authors - Tianrong Xun ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (06-28-2023)

Good for wb

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

HDAC2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:67165-1-Ig)

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011