Anticorps Monoclonal anti-HDAC11

HDAC11 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1G12B5

N° de cat : 67949-1-Ig

Synonymes

FLJ22237, HD11, HDAC11, histone deacetylase 11

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Galerie de données de validation

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  WB analysis using 67949-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 67949-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules SKOV-3, cellules T-47D
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67949-1-Ig cible HDAC11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC11 Protéine recombinante Ag28335
• Nom complet: histone deacetylase 11
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC009676
• Symbole du gène: HDAC11
• Identification du gène (NCBI): 79885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa.

Publications

Species	Application	Title
human	IHC	Transl Lung Cancer Res; Clinicopathological characteristics and prognostic significance of HDAC11 protein expression in non-small cell lung cancer: a retrospective study.; Authors - Di Lu
human	WB	Cell Discov; Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance; Authors - Yan He