Anticorps Monoclonal anti-HDAC1

HDAC1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

4C4G7

Avis (2)

N° de cat : 66085-1-Ig

Synonymes

GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Galerie de données de validation

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  WB analysis using 66085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 66085-1-Ig

  Western blot analysis of HDAC1 in various cell lines using Proteintech antibody 66085-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66085-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 66085-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66085-1-Ig(HDAC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 66085-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer de la prostate humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:6000-1:24000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

66085-1-Ig cible HDAC1 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC1 Protéine recombinante Ag19128
• Nom complet: histone deacetylase 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC000301
• Symbole du gène: HDAC1
• Identification du gène (NCBI): 3065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

Publications

Species	Application	Title
mouse	WB	Immunity; Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.; Authors - Moumita Datta
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
human	WB	Nat Commun; ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.; Authors - Lifang Li
mouse	WB	Br J Pharmacol; Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation.; Authors - Xiaoyun Zhu
human	ChIP	Front Cell Dev Biol; LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6.; Authors - Huihua Kai
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (06-28-2023)

Excellent antibody for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

HDAC1 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66085-1-Ig)

FH

Andrea (Verified Customer) (01-11-2023)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

HDAC1 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:66085-1-Ig)