Anticorps Monoclonal anti-HADHA

• Phare
• Validé par KD/KO

HADHA Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

3E9B1

N° de cat : 60250-1-Ig

Synonymes

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

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Galerie de données de validation

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  WB analysis of HEK293 cells using 60250-1-Ig

  WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells.

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  WB analysis of A375 using 60250-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60250-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60250-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK293
• Résultats positifs en IHC: tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

60250-1-Ig cible HADHA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HADHA Protéine recombinante Ag1211
• Nom complet: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC009235
• Symbole du gène: HADHA
• Identification du gène (NCBI): 3030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Publications

Species	Application	Title
mouse	WB	Commun Biol; A G1528C Hadha knock-in mouse model recapitulates aspects of human clinical phenotypes for long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency; Authors - Garen Gaston
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Proteomics; Quantitative Proteomic Study of Myocardial Mitochondria in Urea Transporter B Knockout Mice.; Authors - Yanwei Du ; KO Validated
human	WB	Cell Biosci; Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells.; Authors - Shuang Li
mouse	WB	Science; Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice; Authors - Muna Al-Habsi
mouse	WB	RSC Adv; Glycolytic and lipid oxidative metabolic programs are essential for freshly-isolated regulatory T cells in mice with sepsis; Authors - Xiaomei Zhu