Anticorps Recombinant de lapin anti-HA Tag

HA Tag Recombinant Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

6I21

N° de cat : 81290-1-RR

Synonymes

HA, HA Tag

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Galerie de données de validation

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  WB analysis using 81290-1-RR

  HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of Transfected HEK-293T using 81290-1-RR

  IP result of anti-HA Tag(IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:20000) with Transfected HEK-293T cells lysate 795 ug.

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  IF Staining of Transfected HEK-293 using 81290-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:750 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 transfectées,
• Résultats positifs en IP: Transfected HEK-293T cells,
• Résultats positifs en IF: cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:375-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

81290-1-RR cible HA Tag dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: HA Tag
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
	WB	Mol Nutr Food Res; Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer; Authors - Da Huang
		Res Sq; VprBP/DCAF1 triggers melanomagenic gene silencing through histone H2A phosphorylation; Authors - Yonghwan Shin
	WB	Int J Biol Sci; Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways; Authors - Xinyuan Zhao
mouse	WB	Front Cell Dev Biol; EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO; Authors - Pablo Barbeito