Anticorps Monoclonal anti-Glucocorticoid receptor

Glucocorticoid receptor Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D9A9

Avis (1)

N° de cat : 66904-1-Ig

Synonymes

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

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Galerie de données de validation

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  WB analysis using 66904-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 66904-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66904-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66904-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66904-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66904-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66904-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66904-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66904-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66904-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66904-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66904-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66904-1-Ig cible Glucocorticoid receptor dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glucocorticoid receptor Protéine recombinante Ag28431
• Nom complet: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC015610
• Symbole du gène: NR3C1
• Identification du gène (NCBI): 2908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Publications

Species	Application	Title
mouse	WB,IHC	Antioxidants (Basel); Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress; Authors - Wenji Ren
mouse	WB	Am J Physiol Cell Physiol; Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.; Authors - Lisa M Cooper
mouse	IF	Antioxidants (Basel); Dim Blue Light at Night Induces Spatial Memory Impairment in Mice by Hippocampal Neuroinflammation and Oxidative Stress; Authors - Qi Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shubham (Verified Customer) (04-15-2019)

Good

• Applications: Western Blot,
• Cell Tissue Type: AC16

Glucocorticoid receptor Antibody Western Blot, validation ( dilution) in AC16 (Cat no:66904-1-Ig)