Anticorps Monoclonal anti-GUCY2C

GUCY2C Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

1F5F6

N° de cat : 67782-1-Ig

Synonymes

GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR

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Galerie de données de validation

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  WB analysis of Caco-2 using 67782-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HaCaT using 67782-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67782-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HaCaT, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67782-1-Ig cible GUCY2C dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GUCY2C Protéine recombinante Ag19256
• Nom complet: guanylate cyclase 2C (heat stable enterotoxin receptor)
• Masse moléculaire calculée: 1073 aa, 123 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC136544
• Symbole du gène: GUCY2C
• Identification du gène (NCBI): 2984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GUCY2C is also named as GUC2C, STAR(Heat-stable enterotoxin receptor), Guanylyl cyclase C and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a receptor for the E.coli heat-stable enterotoxin and considered to be the true apical membrane receptor for the endogenous ligands uroguanylin and guanylin. GUCY2C is not present in the endocrine pancreas, but is localized exclusively to the ductal system which fully corresponds to the GUCY2C activity detected in the exocrine ductal system, but particularly not in islets of Langerhans. GUCY2C with a molecular mass of 130 kDa is detected in pancreatic and intestinal extracts.(PMID:11431160). The full length protein is 123 kDa and has a signal peptide and nine glycosylation sites.