Anticorps Monoclonal anti-GSTP1

• Phare
• Validé par KD/KO

GSTP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2D4A6

N° de cat : 66715-1-Ig

Synonymes

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

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Galerie de données de validation

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66715-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66715-1-Ig

  WB result of GSTP1 antibody (66715-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

66715-1-Ig cible GSTP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSTP1 Protéine recombinante Ag8880
• Nom complet: glutathione S-transferase pi 1
• Masse moléculaire calculée: 210 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC010915
• Symbole du gène: GSTP1
• Identification du gène (NCBI): 2950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng ; KD Validated
human	WB	Bioorg Chem; Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1; Authors - Qiu-Lin Yan
rat	WB	Free Radic Biol Med; Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1; Authors - Huimin Zhu
human	WB	Cell Mol Life Sci; NUMA1 modulates apoptosis of esophageal squamous cell carcinoma cells through regulating ASK1-JNK signaling pathway; Authors - Shuying Yin ; KD Validated