Anticorps Monoclonal anti-GSTM1

GSTM1 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, poulet, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C5C2

N° de cat : 67862-1-Ig

Synonymes

glutathione S transferase mu 1, GST class mu 1, GST HB subunit 4, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, MU, MU 1

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Galerie de données de validation

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  WB analysis of rat liver using 67862-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 67862-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67862-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67862-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 67862-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of chicken liver using 67862-1-Ig

  chicken liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 67862-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GSTM1 (67862-1-Ig, Clone:3C5C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules HeLa, cellules Jurkat, tissu hépatique de porc, tissu hépatique de poulet, tissu hépatique de souris
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67862-1-Ig cible GSTM1 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, poulet, rat, souris

• Réactivité: Humain, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSTM1 Protéine recombinante Ag3113
• Nom complet: glutathione S-transferase mu 1
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC024005
• Symbole du gène: GSTM1
• Identification du gène (NCBI): 2944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.