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Anticorps Monoclonal anti-GSK3B

GSK3B Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2E9E4

N° de cat : CL594-67329

Synonymes

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GSK3B Monoclonal antibody (CL594-67329)

    IF Staining of HeLa using CL594-67329

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GSK3B antibody (CL594-67329, Clone: 2E9E4 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated GSK3B Monoclonal antibody (CL594-67329)

    FC experiment of HeLa using CL594-67329

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GSK3B (CL594-67329, Clone:2E9E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67329 cible GSK3B dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène GSK3B Protéine recombinante Ag17320
Nom complet glycogen synthase kinase 3 beta
Masse moléculaire calculée 433 aa, 48 kDa
Poids moléculaire observé 46-48 kDa
Numéro d’acquisition GenBankBC000251
Symbole du gène GSK3B
Identification du gène (NCBI) 2932
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Protocole

Product Specific Protocols
IF protocol for CL594 GSK3B antibody CL594-67329Download protocol
FC protocol for CL594 GSK3B antibody CL594-67329Download protocol
Standard Protocols
Click here to view our Standard Protocols