Anticorps Monoclonal anti-GPNMB

GPNMB Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2B10B8

N° de cat : 66926-1-Ig

Synonymes

GPNMB, HGFIN, Transmembrane glycoprotein NMB

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Galerie de données de validation

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  WB analysis using 66926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66926-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human liver using 66926-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HeLa using 66926-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: SK-BR-3 cells, cellules A375, cellules HeLa, cellules HT-1080, cellules PC-12, cellules RAW 264.7, cellules THP-1, cellules U-251, cellules U-937
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66926-1-Ig cible GPNMB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPNMB Protéine recombinante Ag26747
• Nom complet: glycoprotein (transmembrane) nmb
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC011595
• Symbole du gène: GPNMB
• Identification du gène (NCBI): 10457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Publications

Species	Application	Title
human	WB,IHC,IF	Int Immunopharmacol; Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma; Authors - Ying Lin
mouse	WB,IF	Int Immunopharmacol; PCSK9 inhibitor protects against ischemic cerebral injury by attenuating inflammation via the GPNMB/CD44 pathway; Authors - Yaling Zheng
human,mouse	WB,IHC	Cell Signal; A comprehensive evaluation of circ_0065214/ miR-188-3p/GPNMB axis in breast cancer; Authors - Xi-Xi Wu
human	IHC	Cancers (Basel); TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma; Authors - Soji Toda