Anticorps Monoclonal anti-GP73/GOLPH2

GP73/GOLPH2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3D1F1

N° de cat : CL594-66331

Synonymes

bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, GP73/GOLPH2, PSEC0257, UNQ686/PRO1326

---

Galerie de données de validation

• 

  IF Staining of HeLa using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66331 (GP73/GOLPH2 antibody) at dilution of 1:100.

• 

  IF Staining of HeLa using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:400.

• 

  IF Staining of HeLa using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:400.

• 

  IF Staining of HeLa using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:800.

• 

  IF Staining of HeLa using CL594-66331

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:800.

• 

  FC experiment of HeLa using CL594-66331

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GP73/GOLPH2 (CL594-66331, Clone:3D1F1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66331 cible GP73/GOLPH2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GP73/GOLPH2 Protéine recombinante Ag7207
• Nom complet: golgi membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC001740
• Symbole du gène: GOLM1
• Identification du gène (NCBI): 51280
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C