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Anticorps Monoclonal anti-GORASP2

GORASP2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9A3

N° de cat : CL488-66627

Synonymes

DKFZp434D156, FLJ13139, Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GORASP2 Monoclonal a (CL488-66627)

    IF Staining of HepG2 using CL488-66627

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GORASP2 antibody (CL488-66627, Clone: 1C9A3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated GORASP2 Monoclonal a (CL488-66627)

    FC experiment of HepG2 using CL488-66627

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GORASP2 (CL488-66627, Clone:1C9A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66627 cible GORASP2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat

Réactivité Humain, porc, rat
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène GORASP2 Protéine recombinante Ag17607
Nom complet golgi reassembly stacking protein 2, 55kDa
Masse moléculaire calculée 55 kDa
Poids moléculaire observé 59 kDa
Numéro d’acquisition GenBankBC007770
Symbole du gène GORASP2
Identification du gène (NCBI) 26003
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 GORASP2 antibody CL488-66627Download protocol
FC protocol for CL Plus 488 GORASP2 antibody CL488-66627Download protocol
Standard Protocols
Click here to view our Standard Protocols