• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GMAP-210

GMAP-210 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 26456-1-AP

Synonymes

CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230



Applications testées

Résultats positifs en WBcellules HeLa, cellules HL-60
Résultats positifs en IFcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

26456-1-AP cible GMAP-210 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GMAP-210 Protéine recombinante Ag13867
Nom complet thyroid hormone receptor interactor 11
Masse moléculaire calculée 1979 aa, 228 kDa
Poids moléculaire observé 230 kDa
Numéro d’acquisition GenBankBC002656
Symbole du gène TRIP11
Identification du gène (NCBI) 9321
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.

Protocole

Product Specific Protocols
WB protocol for GMAP-210 antibody 26456-1-APDownload protocol
IF protocol for GMAP-210 antibody 26456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Biol Chem

Tumor protein D54 binds intracellular nanovesicles via an extended amphipathic region.

Authors - Antoine Reynaud
humanWB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
humanWB,IF

Hum Mutat

Biallelic deep intronic variant c.5457+81T>A in TRIP11 causes loss of function and results in achondrogenesis 1A.

Authors - Priyanka Upadhyai
humanWB

Biol Open

GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.

Authors - Jennifer Y Liu
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

  • Applications: Western Blot
FH

Yuki (Verified Customer) (01-24-2023)

I will recommend this antibody for IF

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: 293T
GMAP-210 Antibody Immunofluorescence validation (1:250 dilution) in 293T (Cat no:26456-1-AP)