Anticorps Monoclonal anti-GLUT4

GLUT4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

73

CloneNo.

3G7C9

Avis (1)

N° de cat : 66846-1-Ig

Synonymes

GLUT 4, GLUT4, SLC2A4

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Galerie de données de validation

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  WB analysis using 66846-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66846-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66846-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66846-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 66846-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66846-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 66846-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 66846-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HEK-293 using 66846-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GLUT4 (66846-1-Ig, Clone:3G7C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, cellules C2C12, tissu adipeux de souris, tissu cardiaque de porc, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 68 publications below
• IHC: See 5 publications below
• IF: See 7 publications below

Informations sur le produit

66846-1-Ig cible GLUT4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GLUT4 Protéine recombinante Ag15390
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 4
• Masse moléculaire calculée: 509 aa, 55 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC069615
• Symbole du gène: SLC2A4
• Identification du gène (NCBI): 6517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an INS-dependent manner.

What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?

The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).

What is the subcellular localization of GLUT4?

Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with  INS - in basal conditions GLUT4 is retained intracellularly, while upon  INS stimulation it is translocated to the plasma membrane (PMID: 18570632).

What molecules can be transported by GLUT4?

Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.

What is the tissue expression pattern of GLUT4?

GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to INS.

Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?

Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to  INS(PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).

Publications

Species	Application	Title
human	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
human	WB	Adv Sci (Weinh); NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer; Authors - Xiaona Chen
mouse	WB,IHC	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
human	WB	Oncogene; Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.; Authors - Shaojia Wang
mouse	WB	Oxid Med Cell Longev; Total Sesquiterpene Glycosides from Loquat Leaves Ameliorate HFD-Induced Insulin Resistance by Modulating IRS-1/GLUT4, TRPV1, and SIRT6/Nrf2 Signaling Pathways.; Authors - Ruoyun Wu
mouse	WB	Oxid Med Cell Longev; Protective Effect of Jiang Tang Xiao Ke Granules against Skeletal Muscle IR via Activation of the AMPK/SIRT1/PGC-1α Signaling Pathway.; Authors - Ying Bai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (09-07-2023)

Good antibody for WB. Signal is strong, and the background is clean. It seems not work with GLUT4 of mouse origin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 or 1:2000
• Cell Tissue Type: human muscle, human heart, mouse liver, heart, muscle, and brain.

GLUT4 Antibody Western Blot validation (1:1000 or 1:2000 dilution) in human muscle, human heart, mouse liver, heart, muscle, and brain. (Cat no:66846-1-Ig)